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Molecular Biology Applications
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DNA extraction |
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from blood (volumes from 1 µl up to 10 ml) |
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from animal and plant tissues |
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from bacterial and fungal cultures |
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RNA extraction from blood, cell lines, tissues and cDNA synthesis |
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de novo gene synthesis |
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sequence design and optimisation, codon usage adaptation, assembly of genes up to several kb, 100% sequence accuracy |
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subcloning in any suitable vector |
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genome libraries |
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in vitro mutagenesis |
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site directed mutagenesis, base substitutions, deletions, insertions of several bases in parallel |
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introduction of multiple mutations in one template |
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random mutagenesis |
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error-prone PCR |
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Exon sequencing |
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detection of mutations by the use of PCR and subsequent DNA sequencing |
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SNP analysis |
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DNA cloning projects |
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Miscellaneous PCR techniques |
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(e.g. TAIL PCR, inverse PCR, RT PCR etc.) |
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Different kinds of projects in molecular biology |
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Micro satellite analysis of human, animal, and plant samples in single or multiplex assays |
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SNP analyses of human, animal and plant samples |
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Expression analyses |
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RealTime PCR |
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Genotyping using micro satellites, RFLP, SNP, DNA sequencing |
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Research and Development Projects, Clinical Studies
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Next Generation Sequencing on Genome Sequencer GS 20 and GS FLX |
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High throughput purification / preparation of: |
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PCR products, plasmids, cosmids, BACs |
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High throughput sequencing of: |
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PCR products, plasmids, cosmidc, BACs |
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Whole genome sequencing |
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incl. library construction and assembly of sequencing data |
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Construction of shotgun libraries (even security level 2 organisms possible): |
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shotgun libraries in plasmids derived form genomic DNA and from BACs, PACs or cosmids |
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shotgun libraries in cosmids |
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cDNA services |
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construction of cDNA libraries |
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in plasmids and in lambda |
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isolation and cloning of full-length cDNAs |
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5´- and 3´-RACE |
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Mega- and giga-scale isolation of plasmids (even endotoxin-free) |
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DNA-/RNA-isolation for clinical studies |
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isolation of genomic DNA from whole blood |
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isolation of RNA from whole blood |
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isolation of RNA from leukocytes, e.g. PBMCs |
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haemogram / cell number analysis via haematology analyser |
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GLP compliant sequencing |
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DNA Forensics, Applied DNA Testing, Veterinary Analytics, Genotyping, Food Analysis
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DNA extraction |
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from blood (from 1 µl to 10 ml) |
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from buccal swabs, bones, teeth, hairs, feathers, semen, cell lines, tissues (meat), mouse biopsies |
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from trace material, e.g. chewing gum, cigarettes, stamps, envelopes |
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RNA extraction from blood, cell lines, tissue, and cDNA synthesis |
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Micro satellite analysis of human, animal, and plant samples in single or multiplex assays |
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Applications: verification of identity, paternity and parentage testing, linkage analysis, QTL |
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SNP analysis of human, animal and plant samples |
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Applications: verification of identity, paternity and parentage testing, linkage analysis |
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Genotyping using micro satellites, RFLP, SNP, DNA sequencing |
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Sex determination of birds |
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Forensics |
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Breeder services, determination of identity and paternity |
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Food quality controls |
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e.g. traceability of contaminations in food or product development |
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Sex determination of cattle |
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e.g. for the quality control of meat |
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Paternity and parentage testing |
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Genetic tests |
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test for genetic risk of osteoporosis, thrombosis, etc. |
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other panels in preparation |
Technological Know-how